Protein engineering

Posted on Posted in Protein engineering

Overview

Our protein engineering services are currently offered for microbial expression systems (E.coli, yeast), and include protein expression, optimizations, functional characterization, and pilot-scale (10L) bioreactor manufacturing. We use proprietary RecombiPure technology to enable efficient expression of hard or impossible to express proteins.

Capabilities and services

Expression
  • Gene design
  • Pilot expression (E.coli, yeast)
  • Derivation of stable expressing clone
  • Expression optimization
  • Manufacturing
  • Conjugations (fluorophore labeling, etc.)
Characterization
  • Standard assays (yield quantification, SDS-PAGE, western blot, mass spec)
  • Protein-target interaction studies
  • Bioactivity assays
  • Stability studies
Downstream applications